检出限
化学
磁性纳米粒子
核酸酶
荧光
生物传感器
肺癌
劈理(地质)
生物物理学
DNA
纳米技术
纳米颗粒
材料科学
生物
色谱法
生物化学
医学
病理
物理
量子力学
断裂(地质)
复合材料
作者
Qing Liu,Jingjian Liu,Na He,Moli Zhang,Lun Wu,Xiyu Chen,Jun Zhu,Fengying Ran,Qinhua Chen,Hua Zhang
出处
期刊:Molecules
[MDPI AG]
日期:2022-08-22
卷期号:27 (16): 5338-5338
被引量:10
标识
DOI:10.3390/molecules27165338
摘要
Exosomal MicroRNA-21 (miRNA-21, miR-21) is significantly up-regulated in blood samples of patients with lung cancer. Exosomal-derived miR-21 can be used as a promising biomarker for the early diagnosis of lung cancer. This paper develops a fluorescent biosensor based on the combination of magnetic nanoparticles (MNPs), cascade strand displacement reaction (CSDR) and CRISPR/Cas12a to detect the exosomal miR-21 from lung cancer. The powerful separation performance of MNPs can eliminate the potential interference of matrix and reduce the background signal, which is very beneficial for the improvement of specificity and sensitivity. The CSDR can specifically transform one miR-21 into plenty of DNA which can specifically trigger the trans-cleavage nuclease activity of Cas12a, resulting in the cleavage of ssDNA bi-labeled with fluorescent and a quencher. Under the optimized experimental conditions, the developed fluorescence biosensor exhibited high sensitivity and specificity towards the determination of exosomal-derived miR-21 with a linear range from 10 to 1 × 105 fM and a low detection limit of about 0.89 fM. Most importantly, this method can be successfully applied to distinguish the exosomal miR-21 from the lung cancer patients and the healthy people.
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