戊二醛
米曲霉
锌
化学
固定化酶
水解
乳糖
纳米颗粒
酶分析
核化学
酶
色谱法
生物化学
有机化学
材料科学
纳米技术
作者
Shakeel Ahmed Ansari,Ahmed A. Damanhory
出处
期刊:Heliyon
[Elsevier]
日期:2023-02-01
卷期号:9 (2): e13089-e13089
被引量:12
标识
DOI:10.1016/j.heliyon.2023.e13089
摘要
The current research demonstrates the synthesis of zinc oxide nanoparticles (ZnO-NPs) via green nanotechnology approach (Azatirachta indica leaves). The size of the synthesized ZnO-NPs was confirmed as 27 nm by TEM. Glutaraldehyde was used to modify the surface of the developed ZnO-NPs in order to promote covalent binding of Aspergillus oryzae β-galactosidase. Enzyme activity was achieved as 93% on glutaraldehyde modified ZnO-NPs. The immobilized enzyme exhibited significant enhancement in activity under extreme temperature and pH variations, as compared to the soluble β-galactosidase (SβG). It was further observed that the immobilized enzyme retained 58% activity at 5% galactose concentration. However, under similar experimental conditions, SβG showed 27% activity. Reusability of immobilized enzyme revealed that it retained 89% activity even after fifth repeated use, and hence could be recovered easily by centrifugation for repeated use in biotechnological applications. Batch reactor experiment indicates that the immobilized enzyme displayed 81% and 70% lactose hydrolysis at 50 °C and 60 °C, respectively as compared to 70% and 58% lactose hydrolysis by soluble enzyme under identical conditions after 9 h.
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