生物物理学
细胞骨架
材料科学
超细纤维
肌动蛋白细胞骨架
纳米地形
组织工程
纤维
脚手架
细胞生物学
化学
纳米技术
细胞
生物医学工程
生物
复合材料
生物化学
医学
作者
Ya-Jing Ye,Yifan Xu,Yung-Chi Hou,Da‐Chuan Yin,Dihan Su,Zhihui Zhao
出处
期刊:Biomacromolecules
[American Chemical Society]
日期:2023-11-28
卷期号:24 (12): 5859-5870
标识
DOI:10.1021/acs.biomac.3c00896
摘要
Nano scale topography scaffold is more bioactive and biomimetic than smooth fiber topographies. Tendon stem cells (TSCs) play important roles in the tendinogenesis of tendon tissue engineering, but the effects and mechanisms of nano topography on TSC behavior are still unclear. This study determined whether the morphology, proliferation, cytoskeleton, and differentiation of TSCs are affected by topography of scaffold in vitro. The porous PA56 scaffolds were prepared with different concentration ratios of glycerol as the molecular template by electrospinning. Its topological characteristics, hydrophilicity, and degradation properties varied with glycerol proportion and movement rate of the receiving plate. Porous fibers promoted the proliferation of TSCs and the number of TSCs varied with topography. Although there was no significant difference due to the small sample size, the number of pseudopodia and cell polarizability still showed differences among different topographies. The morphology of actin cytoskeleton of TSCs showed difference among cultured on porous fibers, smooth fibers, and in culture media with no fiber, suggesting the orientation growth of cells on porous fiber. Moreover, porous fibers promoted teno-lineage differentiation of TSCs by upregulating tendon-specific gene expression. These findings provide evidence that nano porous topography scaffold promotes TSC proliferation, cytoskeleton orientation, and tenogenic differentiation.
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