颗粒酶B
转移
细胞因子
细胞毒性
免疫系统
生物
微生物学
先天免疫系统
刺激
免疫学
癌症研究
化学
分子生物学
癌症
体外
T细胞
生物化学
内分泌学
遗传学
作者
Minjeong Jo,Jee‐Hoon Jung,Han Wool Kim,Sue Jung Lee,Young Min,Hee Sook Jee,Tae‐Jong Yoon,Kihyun Shin
标识
DOI:10.1016/j.jcs.2020.102919
摘要
To develop novel functional ingredients, a polysaccharide called BF-E2-P was purified from fermented barley and its antitumor activity was investigated. In the experimental lung metastasis model of colon 26-M3.1 carcinoma cells, intravenous administration of BF-E2-P (500 μg/mouse) 2 days before inoculation resulted in inhibition of metastasis by about 76%, and the extent of inhibition was dose-dependent. To demonstrate the anti-metastatic activity of BF-E2-P, we tested whether BF-E2-P could activate the innate immune system. Stimulation of macrophages by BF-E2-P resulted in cytokine production. In a co-culture of macrophages and cancer cells, BF-E2-P altered the pattern of cytokine production, suggesting that BF-E2-P affects the polarization of macrophages. In addition, intravenous administration of BF-E2-P augmented the natural killer (NK) cell-mediated cytotoxicity against cancer cells. It also increased the production of IFN- γ and granzyme B. In conclusion, BF-E2-P activates NK cells and macrophages, resulting in the enhancement of host-defense against tumor growth.
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