dna疫苗
单纯疱疹病毒
病毒学
接种疫苗
启动(农业)
异源的
免疫系统
免疫
免疫学
病毒
生物
抗体
免疫
医学
生物化学
植物
发芽
基因
作者
Rebecca S. Tirabassi,Christopher I. Ace,Tatyana S. Levchenko,Vladimir P. Torchilin,Liisa K. Selin,Siwei Nie,Dennis L. Guberski,Kejian Yang
出处
期刊:Vaccine
[Elsevier]
日期:2011-01-01
卷期号:29 (5): 1090-1098
被引量:12
标识
DOI:10.1016/j.vaccine.2010.11.076
摘要
An estimated 1 out of every 5 Americans is infected with herpes simplex virus type 2 (HSV-2). Efforts in developing a potent vaccine for HSV-2 have shown limited success. Here we describe a heterologous vaccination strategy for HSV-2 based on an intramuscular DNA prime followed by a liposome-encapsulated antigen boost delivered intranasally. Both portions of the vaccine express the immunogenic HSV-2 glycoprotein D. In female Balb/c mice, this heterologous immunisation regimen stimulated high titers of serum neutralising antibodies, a DNA priming dose dependent T helper type response, enhanced mucosal immune responses and potent protective immunity at the portal of entry for the virus: the vaginal cavity. A clear synergistic effect on immune responses and protection from infection was seen using this heterologous immunisation approach. Suboptimal DNA prime (0.5 μg) followed by the liposome boost resulted in an 80% survival rate when mice were infected 2 weeks after immunisation. A higher dose of DNA priming (5 μg) followed by the liposome boost resulted in sterilising immunity in 80% of mice. The vaccine induced durable protection in mice, demonstrated by a 60% survival rate when lethal infections were performed 20 weeks after the immunisation primed with 0.5 μg of DNA vaccine.
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