生物
汉坦病毒
汉坦病毒
双杂交筛选
泛素
汉坦病毒肺综合征
分子生物学
免疫沉淀
病毒学
酵母
细胞生物学
病毒
生物化学
基因
作者
Byoung-Hee Lee,Kumiko Yoshimatsu,Akihiko Maeda,Kazuhiko Ochiai,Masami Morimatsu,Koichi Araki,Michiko Ogino,Shigeru Morikawa,Jiro Arikawa
出处
期刊:Virus Research
[Elsevier]
日期:2003-12-01
卷期号:98 (1): 83-91
被引量:57
标识
DOI:10.1016/j.virusres.2003.09.001
摘要
We performed yeast two-hybrid screening of a human kidney cell cDNA library to study the biological role of the hantavirus nucleocapsid protein (NP). We found that Seoul virus (SEOV) and Hantaan virus (HTNV) NPs were associated with small ubiquitin-like modifier (SUMO)-1-interacting proteins PIAS1, PIASxβ, HIPK2, CHD3, and TTRAP, which interacted with the SUMO-1 conjugating enzyme (Ubc-9) and SUMO-1 in the yeast two-hybrid assay. Interactions between the HIPK2, CHD3, and TTRAP proteins and SEOV NP were also shown in a mammalian two-hybrid assay. However, there was no interaction between PIAS proteins and NP, which was probably due to the inhibitory effect of PIAS on transcription in the mammalian two-hybrid assay. Nevertheless, a co-expression experiment suggested the existence of a PIAS-NP interaction in the cytoplasm. The region spanning amino acids 100–125 of SEOV NP, which represents a critical region for NP–NP polymerization, was found to be responsible for the interaction with SUMO-1-related molecules in both the yeast and mammalian two-hybrid assays. These results add to the information on interactions of hantavirus NP and host cellular proteins.
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