顺铂
细胞培养
基因亚型
金属硫蛋白
分子生物学
生物
亚细胞定位
细胞
谷胱甘肽
细胞毒性T细胞
细胞生物学
癌症研究
化学
作者
Yukihiro Kondo,Shiu-Ming Kuo,Simon C. Watkins,John S. Lazo
出处
期刊:Cancer Research
[American Association for Cancer Research]
日期:1995-02-01
卷期号:55 (3): 474-7
被引量:46
摘要
Metallothioneins (MT) are major cysteine-rich proteins with poorly characterized functions. We have examined the MT amount, isotype expression, and subcellular distribution in 4 human hormone-independent prostatic carcinoma cell lines. Both PC-3 and DU-145 cells were thiol-rich cells with similar MT and glutathione levels, while HPC36M and PC-3 MA2 were thiol-poor cells with lower MT and glutathione levels. All 4 prostatic cell lines expressed the MTIIA isoform at a basal level; DU-145 cells also constitutively expressed MTIE mRNA. Using antibodies for both total MT and MTIIA, we defined MT to cytoplasmic and nuclear domains in PC-3 cells, to perinuclear and nuclear domains in HPC36M cells, and to prominent nonnucleolar nuclear domains in DU-145 and PC-3 MA2 cells. These results indicate that the subcellular distribution is cell type specific and not reflective of the total MT content or MT isoform. Resistance to cadmium in all 4 cell lines was correlated with total MT levels, while resistance to the anticancer agent cisplatin correlated best with nuclear MT content. We suggest that the subcellular localization of MT is functionally important in cellular protection against the anticancer agent cisplatin in human prostatic cancer cells.
科研通智能强力驱动
Strongly Powered by AbleSci AI