核受体
转录因子
糖皮质激素受体
核受体辅活化子2
受体
内科学
内分泌学
交易激励
核受体辅活化子3
核受体辅活化子1
激素受体
转录调控
蜕皮激素受体
类固醇激素
抄写(语言学)
转换抑制
激素
作者
Meng Li,Pengcheng Liu,Jessica D. Wiley,Reyhaneh Ojani,David R. Bevan,Jianyong Li,Jinsong Zhu
标识
DOI:10.1016/j.mce.2014.06.021
摘要
Methoprene-tolerant (Met) protein is a juvenile hormone (JH) receptor in insects. JH-bound Met forms a complex with the βFtz-F1-interacting steroid receptor coactivator (FISC) and together they regulate JH response genes in mosquitoes. Both proteins contain basic helix-loop-helix (bHLH) and PAS motifs. Here we demonstrated that FISC is the obligatory partner of Met for binding to JH-response elements (JHREs). Met or FISC alone could not bind a previously characterized JHRE, while formation of the Met-FISC complex was necessary and sufficient to bind to the JHRE. This binding required participation of the DNA-binding domains of both Met and FISC. The optimal DNA sequence recognized by Met and FISC contained a core consensus sequence GCACGTG. While formation of the Met-FISC complex in mosquito cells was induced by JH, heterodimerization and DNA binding of bacterially expressed Met and FISC were JH-independent, implying that additional mosquito proteins were required to modulate formation of the receptor complex.
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