The Application of Tissue-Culture Methods to the Study of Poliomyelitis

PROGRESS toward the control or understanding of the epidemiology of a specific infectious disease has often been associated with the development of a new procedure for the isolation or cultivation of the etiologic agent. An example of the effect of such a technologic advance is apparent in the field of research on poliomyelitis. In 19491 , 2 it was demonstrated that the poliomyelitis viruses could be propagated in tissue cultures composed of nonnervous human tissues. Since then, the application of tissue-culture methods has resulted in the development of simple and economical procedures for the isolation of the poliomyelitis viruses and for the . . .