清醒
神经科学
臂旁外侧核
臂旁核
光遗传学
谷氨酸的
核心
光刺激
红藻氨酸
内分泌学
内科学
下丘脑
外侧下丘脑
生物
唤醒
桥
作者
Chang-Rui Chen,Yu-Heng Zhong,Shan Jiang,Wei Xu,Lei Xiao,Zan Wang,Wei-Min Qu,Zhi-Li Huang
出处
期刊:eLife
[eLife Sciences Publications, Ltd.]
日期:2021-11-17
卷期号:10
被引量:1
摘要
Hypersomnolence disorder (HD) is characterized by excessive sleep, which is a common sequela following stroke, infection or tumorigenesis. HD is traditionally thought to be associated with lesions of wake-promoting nuclei. However, lesions of a single wake-promoting nucleus, or even two simultaneously, did not exert serious HD. Therefore, the specific nucleus and neural circuitry for HD remain unknown. Here, we observed that the paraventricular nucleus of the hypothalamus (PVH) exhibited higher c-fos expression during the active period (23:00) than during the inactive period (11:00) in mice. Therefore, we speculated that the PVH, in which most neurons are glutamatergic, may represent one of the key arousal-controlling centers. By using vesicular glutamate transporter 2 (vglut2 Cre ) mice together with fiber photometry, multichannel electrophysiological recordings, and genetic approaches, we found that PVH vglut2 neurons were most active during wakefulness. Chemogenetic activation of PVH vglut2 neurons induced wakefulness for 9 h, and photostimulation of PVH vglut2 →parabrachial complex/ventral lateral septum circuits immediately drove transitions from sleep to wakefulness. Moreover, lesioning or chemogenetic inhibition of PVH vglut2 neurons dramatically decreased wakefulness. These results indicate that the PVH is critical for arousal promotion and maintenance.
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