药物输送
细胞凋亡
波形蛋白
透明质酸
癌细胞
A549电池
肺癌
化学
癌症研究
靶向给药
体外
药理学
生物化学
癌症
纳米技术
材料科学
医学
免疫学
病理
免疫组织化学
内科学
解剖
作者
Yan Ma,Jinli Liu,Xinyu Cui,Jing Hou,Fengbo Yu,Jinghua Wang,Xiaoxue Wang,Cong Chen,Lei Tong
出处
期刊:Molecules
[MDPI AG]
日期:2022-07-17
卷期号:27 (14): 4553-4553
被引量:12
标识
DOI:10.3390/molecules27144553
摘要
Lung cancer seriously threatens the health of human beings, with non-small cell lung cancer (NSCLC) accounting for 80%. Nowadays, the potential position of nano-delivery in treating cancer has been the subject of continuous research. The present research aimed to prepare two molecular weight hyaluronic acid (HA)-modified kaempferol (KA)-loaded nanostructured lipid carriers (HA-KA-NLCs) by the method of melting ultrasonic and electrostatic adsorption, and to assess the antitumor effect of the preparations on A549 cells. The characterization and safety evaluation of the preparations illustrated that they are acceptable for drug delivery for cancer. Subsequently, differential scanning calorimetry (DSC) curve and transmission electron microscopy (TEM) images indicated that the drug was adequately incorporated in the carrier, and the particle appeared as a sphere. Moreover, HA-KA-NLC showed predominant in vitro antitumor effects, inhibiting proliferation, migration, and invasion, promoting apoptosis and increasing cellular uptake of A549 cells. Otherwise, the Western blot assay revealed that preparations could activate epithelial-mesenchymal transition (EMT)-related signaling pathways and modulate the expression of E-cadherin, N-cadherin, and Vimentin in A549 cells. Our present findings demonstrated that HA-KA-NLC could be considered as a secure and effective carrier for targeted tumor delivery and may have potential application prospects in future clinic therapy of NSCLC.
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