氧化应激
小胶质细胞
谷胱甘肽
脂多糖
SH-SY5Y型
化学
抗氧化剂
神经炎症
生物化学
淀粉样蛋白(真菌学)
β淀粉样蛋白
过氧化氢酶
细胞培养
分子生物学
药理学
内分泌学
生物
炎症
免疫学
酶
肽
无机化学
遗传学
神经母细胞瘤
作者
Benita Wiatrak,Piotr Jawień,Agnieszka Matuszewska,Adam Szeląg,Adriana Kubis-Kubiak
标识
DOI:10.1016/j.biopha.2022.112880
摘要
Amyloid deposits and hyperphosphorylation of the tau protein are still believed to be the two main causes of Alzheimer's disease. However, newer studies show the beneficial (including antiradical and antimicrobial) effects of amyloid at physiological concentrations. Therefore, this study aimed to investigate the impact of three amyloid fragments - 25-35, 1-40, and 1-42 at concentrations close to physiological levels on the oxidative stress induced by the administration of lipopolysaccharide (LPS) or co-culturing with microglia cells. Differentiated SH-SY5Y cells were used, constituting a model of neuronal cells that were preincubated with LPS or supernatant collected from THP-1 cell culture. The cells were treated with amyloid-β fragments at concentrations of 0.001, 0.1, and 1.0 µM, and then biological assays were carried out. The results of the study support the antioxidant properties of Aβ, which may protect neurons from the damaging effects of neuroinflammation. All tested amyloid-β fragments reduced oxidative stress and increased the levels of enzymatic stress parameters - the activity of SOD, GPx and catalase. In addition, the administration of amyloid-β at low physiological concentrations also increased reduced glutathione (GSH) levels and the ratio between reduced and oxidized glutathione (GSH/GSSG), which is considered a good indicator of maintaining cellular redox balance. Furthermore, a stronger antioxidant effect of 1-40 fragment was observed, occurring in a wider range of concentrations, compared to the other tested fragments 25-35 and 1-42.
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