医学
兰克尔
骨保护素
强直性脊柱炎
内科学
成骨细胞
内分泌学
刺激
破骨细胞
白细胞介素17
骨髓
骨吸收
骨重建
炎症
受体
体外
激活剂(遗传学)
化学
生物化学
作者
Zizhong Liu,Feng Huang,Gui Luo,Yiwen Wang,Ruikai Du,Weijia Sun,Jianwei Li,Xinxin Yuan,Dengchao Cao,Yuheng Li,Caizhi Liu,Shuai Liang,Xiaoyan Jin,Shukuan Ling,Deqing Wang,Yingxian Li
出处
期刊:Rheumatology
[Oxford University Press]
日期:2019-11-13
卷期号:59 (5): 1159-1169
被引量:18
标识
DOI:10.1093/rheumatology/kez594
摘要
Abstract Objective Bone loss is common in AS, and miR-214 plays an important role in regulating bone formation. The aim of this study was to investigate the effect of miR-214, the production of which is stimulated by IL-17A, on bone loss in AS. Methods Peripheral blood was obtained from 32 patients with AS and 24 healthy controls. Levels of IL-17A, soluble RANK ligand (RANKL) and osteoprotegerin in serum were evaluated by ELISA, and the relative level of miR-214 in serum was detected by real-time quantitative PCR. In addition, we assessed the relationship between levels of miR-214, IL-17A and bone loss in primary murine osteoblasts and mouse bone marrow cells. Results The expression of RANKL and miR-214 in osteoblasts was increased following stimulation by IL-17A, and osteoblasts stimulated by IL-17A promoted the expression of miR-214 in osteoclasts and the activity of osteoclasts. We showed that osteoblast-derived miR-214 could be transferred to osteoclasts and could then regulate their activity. The levels of IL-17A and miR-214 were much higher in the serum of patients with AS than in that of healthy controls, and the relative level of miR-214 was positively correlated with the level of IL-17A in the serum and synovial fluid of the patients with AS, not healthy controls. The level of miR-214 in the serum of AS patients has potential diagnostic value. Conclusion The production of miR-214 in osteoblasts is stimulated by IL-17A. It is an important inhibitor of bone formation in AS, and the serum level of miR-214 might be of potential diagnostic value for AS.
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