[Effect of Quercetin on Proliferation and Apoptosis of Multiple Myeloma Cells and Its Related Mechanism].

To investigate the effect of quercetin (que) on proliferation and apoptosis of multiple myeloma cell line NCI-H929.NCI-H929 cells were routinely cultured, and cells in logarithmic growth phase were taken and used for experiments. After treatment of NCI-H929 cells with Que of 50, 100 and 200 µmol/ L for 24, 48 and 72 hours, the proliferation level of cells was detected by using MTT method; after treatment of NCI-H929 cells with Que of 100 and 200 µmol/ L for 24 hours, the cell apoptosis level was detected by Annexin V-FITC/PI double staining, the changes of cell cycle was analysis by flow cytometry with PI marking; the expression of apoptosis-related proteins caspase-3, caspase-8, caspase-9, PARP, BCL-2 and cell cycle-related proteins P53, P21, P27, CDK4, and the activiation of ERK and ATK were detected by Western blot.Que of different concentration could inhibit cell proliferation with time and dose dependent manner. The flow cytometry showed that Que could significantly increase the cell apoptosis and arrest NCI-H929 cells in the G2/M phase. In addition, Western blot analysis showed that Que could activate the apoptosis-related proteins, such as caspase-3, caspase-8, caspase-9 and PARP, and then inhibited the expression of BCL-2. Que could promote the expression of P53, P21 and P27, however, it could inhibited the CDK4 expression in NCI-H929 cells. Que could decrease the phosphorylation levels of p-ERK and p-AKT in NCI-H929 cells.Quercetin mediates anti-myeloma effects through inducing apoptosis, cell cycle arrest and down-regulating ERK and AKT pathways in human myeloma cells.槲皮素对多发性骨髓瘤的抗肿瘤作用及其相关机制.研究槲皮素对多发性骨髓瘤细胞NCI-H929增殖、凋亡、周期的影响及其作用机制.常规培养NCI-H929细胞，取对数生长期的细胞进行实验。用不同浓度槲皮素（50、100、200 µmol/L）处理NCI-H929细胞24、48、72 h后，采用MTT法检测细胞增殖情况；用槲皮素100、200 µmol/ L处理NCI-H929细胞24 h后，使用Annexin V-FITC/PI试剂盒检测细胞凋亡水平，采用PI标记流式细胞术分析细胞周期的变化；采用Western blot法分析凋亡相关蛋白caspase-3、caspase-8、caspase-9、PARP、BCL-2和细胞周期相关蛋白P53、 P21、P27、CDK4蛋白的表达水平，并检测ERK和AKT的活化情况.不同浓度的槲皮素可显著抑制NCI-H929细胞的增殖，抑制效应随时间的延长和剂量的增大而增强。流式细胞术分析结果显示，槲皮素可显著诱导NCI-H929细胞凋亡，并诱导细胞发生G2/M期阻滞。Western blot实验结果显示，槲皮素能够活化caspase-3、caspase-8、caspase-9和PARP，进而抑制BCL-2的表达，促进P53、P21、P27蛋白的表达和抑制CDK4蛋白的表达，并降低ERK、AKT磷酸化水平.槲皮素可有效发挥对骨髓瘤细胞NCI-H929的抗肿瘤作用，该作用可能是通过诱导细胞凋亡、促进细胞周期阻滞和下调ERK/AKT通路实现的.