生物
星形胶质细胞
免疫标记
共域化
胶质纤维酸性蛋白
共焦
免疫染色
神经科学
细胞生物学
解剖
中枢神经系统
免疫组织化学
免疫学
几何学
数学
作者
Kamel Kacem,Pierre Lacombe,Jacques Seylaz,Gilles Bonvento
出处
期刊:Glia
[Wiley]
日期:1998-05-01
卷期号:23 (1): 1-10
被引量:313
标识
DOI:10.1002/(sici)1098-1136(199805)23:1<1::aid-glia1>3.0.co;2-b
摘要
Despite the increasing evidence for a prominent role played by the perivascular endfeet of astrocytes in the functional metabolic coupling between astrocytes and neurons, a clear picture of their spatial organization is still lacking. To examine the three-dimensional structure of the astrocyte endfeet and their relationships with the endothelial cells, coronal rat brain sections immunolabeled for the two astroglial markers [glial fibrillary acidic protein (GFAP)/S-100beta] and the endothelial glucose transporter (GLUT1) were analyzed under the confocal microscope. Double immunolabeling of GFAP and S-100beta showed numerous well-defined astrocytes sending one or more endfeet to the vasculature. Examination of GFAP immunolabeling at higher magnification showed that these endfeet consist of well-defined rosette-like structures lying on the vessel wall. Double immunostaining of GFAP and GLUT1 showed that the endothelial cells were the main targets of these repeated geometrical units formed by the astrocyte endfeet. When three-dimensional images were reconstructed, obvious privileged anatomical relationships were observed between endfeet and individual endothelial cells. These anatomical data provide strong support for the involvement of astrocytes in cerebral metabolic coupling. The finger-like appearance of astrocyte endfeet could allow direct metabolic exchanges between intracerebral vessels and non-glial elements such as nerve terminals.
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