氧离子
糖蛋白组学
糖基化
质谱法
糖肽
糖蛋白
化学
串联质谱法
转铁蛋白
血液蛋白质类
色谱法
离子
聚糖
生物化学
有机化学
抗生素
作者
Matthew White,Ludwig Sinn,D. Marc Jones,Joost de Folter,Simran Kaur Aulakh,Ziyue Wang,Helen Flynn,Lynn Krüger,Pinkus Tober-Lau,Vadim Demichev,Florian Kurth,Michael Mülleder,Véronique Blanchard,Christoph B. Messner,Markus Ralser
标识
DOI:10.1038/s41551-023-01067-5
摘要
Abstract Protein glycosylation, a complex and heterogeneous post-translational modification that is frequently dysregulated in disease, has been difficult to analyse at scale. Here we report a data-independent acquisition technique for the large-scale mass-spectrometric quantification of glycopeptides in plasma samples. The technique, which we named ‘OxoScan-MS’, identifies oxonium ions as glycopeptide fragments and exploits a sliding-quadrupole dimension to generate comprehensive and untargeted oxonium ion maps of precursor masses assigned to fragment ions from non-enriched plasma samples. By applying OxoScan-MS to quantify 1,002 glycopeptide features in the plasma glycoproteomes from patients with COVID-19 and healthy controls, we found that severe COVID-19 induces differential glycosylation in IgA, haptoglobin, transferrin and other disease-relevant plasma glycoproteins. OxoScan-MS may allow for the quantitative mapping of glycoproteomes at the scale of hundreds to thousands of samples.
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