卵巢癌
间质细胞
基质金属蛋白酶
浆液性液体
癌症研究
阶段(地层学)
病理
癌症
癌细胞
医学
体内
化学
生物
内科学
生物技术
古生物学
作者
Kuo Hwa Wang,Yung Ming Wang,Li Hsuan Chiu,Tze Chien Chen,Yu Hui Tsai,Zuo Chen,Kuan Chou Chen,Chun A. Changou,Wen Lai
出处
期刊:PLOS ONE
[Public Library of Science]
日期:2018-02-01
卷期号:13 (2): e0192047-e0192047
被引量:9
标识
DOI:10.1371/journal.pone.0192047
摘要
Epithelial ovarian cancer (EOC) is the seventh most common cancer among women worldwide. The 5-year survival rate for women with EOC is only 30%-50%, which is largely due to the typically late diagnosis of this condition. EOC is difficult to detect in its early stage because of its asymptomatic nature. Recently, near-infrared fluorescent (NIRF) imaging has been developed as a potential tool for detecting EOC at the molecular level. In this study, a NIRF-sensitive probe was designed to detect matrix metalloproteinase (MMP) activity in ovarian cancer cells. A cyanine fluorochrome was conjugated to the amino terminus of a peptide substrate with enzymatic specificity for MMP-3. To analyze the novel MMP-3 probe, an in vivo EOC model was established by subcutaneously implanting SKOV3 cells, a serous-type EOC cell line, in mice. This novel MMP-3-sensitive probe specifically reacted with only the active MMP-3 enzyme, resulting in a significantly enhanced NIRF emission intensity. Histological analysis demonstrated that MMP-3 expression and activity were enhanced in the stromal cells surrounding the ovarian cancer cells. These studies establish a molecular imaging reporter for diagnosing early-stage EOC. Additional studies are required to confirm the early-stage activity of MMP-3 in EOC and its diagnostic and prognostic significance.
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