吲哚试验
异源表达
基因簇
生物信息学
放线菌门
基因
生物化学
生物合成
吲哚生物碱
生物
异源的
计算生物学
生物碱
立体化学
化学
重组DNA
16S核糖体RNA
作者
Xiaokun Xu,Haibo Zhou,Yang Liu,Xiaotong Liu,Jun Fu,Aiying Li,Yuezhong Li,Yuemao Shen,Xiaoying Bian,Youming Zhang
标识
DOI:10.1021/acs.jnatprod.7b00835
摘要
The indole alkaloid antibiotic chuangxinmycin, from Actinobacteria Actinoplanes tsinanensis, containing a unique thiopyrano[4,3,2-cd]indole scaffold, is a potent and selective inhibitor of bacterial tryptophanyl-tRNA synthetase. The chuangxinmycin biosynthetic gene cluster was identified by in silico analysis of the genome sequence, then verified by heterologous expression. Systemic gene inactivation and intermediate identification determined the minimum set of genes for unique thiopyrano[4,3,2-cd]indole formation and the concerted action of a radical S-adenosylmethionine protein plus an unknown protein for addition of the 3-methyl group. These findings set a solid foundation for comprehensively investigating the biosynthesis, optimizing yield, and generating new analogues of chuangxinmycin.
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