Native endophytes from maize as potential biocontrol agents against bacterial top rot caused by cross-kingdom pathogen Klebsiella pneumoniae

生物 病菌 生物病虫害防治 肺炎克雷伯菌 内生菌 微生物学 细菌 作物 人类病原体 园艺 植物 农学 大肠杆菌 遗传学 生物化学 基因
作者
Xiang-Song Li,Pengfei He,Pengbo He,Yongmei Li,Yixin Wu,Chan Mu,Shahzad Munir,Yueqiu He
出处
期刊:Biological Control [Elsevier BV]
卷期号:178: 105131-105131 被引量:5
标识
DOI:10.1016/j.biocontrol.2022.105131
摘要

Maize crop has been suffering huge economic loss due to bacterial top rot disease caused by emerging plant pathogen Klebsiella pneumoniae, which is most concerning and highly contagious to both humans and animals. At present, chemical control is considered as an important disease management approach, however biological control through native endophytes has not been reported yet. Here, initially we isolated 120 native endophytes from maize plants, and screened 12 endophytes with potential antagonistic effect in coculture assay against maize bacterial top rot pathogen. Based on strong antibacterial activity, three endophytes (MZ-4, YN-23, and F14) were selected to evaluate their plant growth-promoting traits and biocontrol efficiency through in-vitro and in-vivo antagonistic assays. The three strains could produce IAA with a maximum yield of 2.54 µg/ml, 2.96 µg/ml, 2.07 µg/ml, respectively and also inhibit the growth of 10 common plant pathogenic bacteria. In planta assay suggested that three endophytes enhanced maize growth and significantly suppressed the incidence of K. pneumoniae with control effect of about 81.2 %, 74.7 %, and 66.7 %, respectively. Lastly, selected isolates MZ-4, YN-23, and F14 were molecularly characterized and identified as Bacillus velezensis. To our knowledge, this is the first study reporting the application of maize endophyte B. velezensis as a biocontrol agent against emerging pathogen of maize bacterial top rot disease.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
爱吃饼干的土拨鼠完成签到,获得积分10
3秒前
6秒前
6秒前
小松鼠完成签到 ,获得积分10
8秒前
9秒前
11秒前
12秒前
丘比特应助杨冰采纳,获得10
12秒前
菜虚鲲发布了新的文献求助10
12秒前
动漫大师发布了新的文献求助20
12秒前
13秒前
肉脸小鱼发布了新的文献求助10
13秒前
自由的雁完成签到,获得积分10
13秒前
kk完成签到,获得积分10
14秒前
CipherSage应助always采纳,获得10
15秒前
16秒前
小小小新完成签到,获得积分10
16秒前
strug783发布了新的文献求助10
17秒前
小小小新发布了新的文献求助10
19秒前
我是老大应助科研通管家采纳,获得10
19秒前
chenyi应助科研通管家采纳,获得10
19秒前
Akim应助科研通管家采纳,获得10
19秒前
19秒前
六十一完成签到,获得积分10
20秒前
木云浅夏发布了新的文献求助10
22秒前
23秒前
南瓜猪猪头完成签到 ,获得积分10
24秒前
25秒前
25秒前
哈哈完成签到,获得积分10
27秒前
xunlei发布了新的文献求助10
27秒前
科目三应助肉脸小鱼采纳,获得10
28秒前
jianglili发布了新的文献求助10
28秒前
Hello应助小小小新采纳,获得10
29秒前
14发布了新的文献求助10
30秒前
31秒前
33秒前
33秒前
34秒前
绝尘发布了新的文献求助10
35秒前
高分求助中
【此为提示信息,请勿应助】请按要求发布求助,避免被关 20000
Encyclopedia of Geology (2nd Edition) 2000
Maneuvering of a Damaged Navy Combatant 650
Периодизация спортивной тренировки. Общая теория и её практическое применение 310
Mixing the elements of mass customisation 300
the MD Anderson Surgical Oncology Manual, Seventh Edition 300
Nucleophilic substitution in azasydnone-modified dinitroanisoles 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3780270
求助须知:如何正确求助?哪些是违规求助? 3325566
关于积分的说明 10223524
捐赠科研通 3040706
什么是DOI,文献DOI怎么找? 1668974
邀请新用户注册赠送积分活动 798936
科研通“疑难数据库(出版商)”最低求助积分说明 758634