马拉特1
PTEN公司
PI3K/AKT/mTOR通路
香豆素
蛋白激酶B
生物
活力测定
癌症研究
免疫印迹
细胞培养
癌症
基因
分子生物学
生物化学
长非编码RNA
信号转导
遗传学
核糖核酸
植物
作者
Shirin Shafaee Arani,Majid Nejati,Sepide Rastgoufar,Arash Raisi,Reza Eshraghi,Amirreza Ostadia,Amir Hassan Matini,Neda Rahimain,Hamed Mirzaei
标识
DOI:10.1016/j.prp.2024.155291
摘要
Because long non-coding RNAs (lncRNAs) can affect several interconnected processes, its value as a predictive marker for gastric cancer has been demonstrated. Coumarin - a natural compound known to contain some beneficial antitumor qualities - was tested for its effects on AGS gastric cancer cells. In this study, we investigated the expression level of selected cellular lncRNAs (BANCR, MALAT1 and FER1L4) and their target genes (PTEN, p-PI3K and p-AKT) in coumarin-treated AGS cell line. The expressions of the three lncRNAs: BANCR, MALAT1 and FER1L4, as well as their specified targets, PTEN, PI3K and AKT, were measured by qRT-PCR. To gauge the impact of coumarin on the AGS cells, a MTT assay was utilized. A Western blot has been employed to assess variations in PTEN, p-PI3K, and p-AKT expression. The experiment's results showed that AGS viability diminished with increasing doses of coumarin. Compared to the control cells, the cells exposed to coumarin had showed reduced levels of mRNAs which are known targets of the lncRNA BANCR. At the same time, levels of lncRNAs MALAT1 and FER1L4 within coumarin group have been higher comparing to those within control group. Additionally, the Western blot analysis revealed that the coumarin-treated cells expressed lower levels of p-PI3K, PTEN as well as p-AKT compared to control group. This information points to coumarin being a possible option in a treatment regimen for gastric cancer due to its ability to affect lncRNAs and the molecules they target.
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