化学
荧光
菁
体内
半胱氨酸
谷胱甘肽
检出限
生物相容性
荧光寿命成像显微镜
选择性
临床前影像学
生物物理学
光化学
生物化学
有机化学
色谱法
酶
物理
生物技术
催化作用
生物
量子力学
作者
Shaolong Qi,Haiyan Zhang,Xinyu Wang,Jialin Lv,Dahai Liu,Wenbin Shen,Yaoxian Li,Jianshi Du,Qingbiao Yang
出处
期刊:Talanta
[Elsevier]
日期:2021-11-01
卷期号:234: 122685-122685
被引量:28
标识
DOI:10.1016/j.talanta.2021.122685
摘要
Cysteine (Cys) plays important physiological roles in the human body, and abnormal Cys concentrations can cause a variety of diseases. Thus, detecting Cys with high selectivity and sensitivity in vivo is important. Near-infrared (NIR) fluorescent probes are widely employed in biological detection because of their excellent optical properties such as minimal damage to biological samples, low background interference and high signal-to-noise ratio. However, few NIR fluorescent probes that can detect Cys over homocysteine (Hcy) and glutathione (GSH) have been reported because of their similar reactivity and structure. In this work, a highly water-soluble NIR probe (CYNA) for detecting Cys whose structure is similar to that of indocyanine green and is based on cyanine skeleton was synthesized and via aromatic nucleophilic substitution–rearrangement (SNAr-rearrangement) to specific recognize the cysteine. The probe showed high selectivity toward Cys and superior biosecurity, excellent biocompatibility and prolonged dynamic imaging. It also has long fluorescence emission wavelength (820 nm), low detection limit (14 nM) and was successfully applied for visualizing Cys in living cells and mice, which has great promise for applications in noninvasive vivo biological imaging and detection.
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