胚状体
胚胎干细胞
中胚层
细胞生物学
干细胞
FGF与中胚层形成
涂层
生物
细胞分化
细胞培养
解剖
材料科学
纳米技术
遗传学
成体干细胞
基因
作者
Derya Sağraç,Selinay Şenkal,Taha Bartu Hayal,Fikrettin Şahın,Zehra Çobandede,Ayşegül Doğan
出处
期刊:Cytotechnology
[Springer Nature]
日期:2022-02-22
卷期号:74 (2): 293-307
被引量:3
标识
DOI:10.1007/s10616-022-00529-z
摘要
Pluripotent stem cells as a promising cell source with unlimited proliferation and differentiation capacity hold great promise for cell-based therapies in regenerative medicine. Establishment of appropriate culture conditions might enable the control of cellular fate decision in cell culture. Transfer of three-dimensional (3D) embryoid bodies to two-dimensional (2D) monolayer culture systems for initiation of cell differentiation and specialization requires an adaptation of cells which can be managed by extracellular matrix (ECM) materials. Here we compare the characteristics of four different cell culture coating materials and their effect on attachment and differentiation of cells spreading from mouse embryonic stem cell (mESC) derived embryoid bodies (EBs) in mesoderm inducing culture conditions. Atomic force microscope (AFM) and scanning electron microscope (SEM) analysis along with Water Contact Angle technique were used to analyze physical properties of ECM materials and to evaluate cellular behavior on surfaces. Cell migration and differentiation were performed initially by using mesoderm inducing culture conditions and then three germ layer specification conditions. We investigated properties of coating materials such as roughness and wettability control cell attachment, migration and differentiation of mESCs. Matrigel-Gelatin combination is suitable for cell attachment and migration of cells spreading from 3D EBs followed by transfer onto coated surfaces. Matrigel-Gelatin coating enhanced differentiation of cells into mesoderm like cells via EMT process. Our data demonstrated that the Matrigel-Gelatin combination as a cell culture coating matrix might serve as a suitable platform to transfer EBs for differentiation and might influence pluripotent stem cell fate decision into mesoderm and further mesoderm derivative cell populations.The online version contains supplementary material available at 10.1007/s10616-022-00529-z.
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