化学
原子转移自由基聚合
聚苯乙烯
甲基丙烯酸酯
吸附
聚合物
阳离子聚合
高分子化学
色谱法
共聚物
溴化物
聚合
基质(化学分析)
化学工程
有机化学
工程类
作者
Jian‐Bo Qu,Yuliang Xu,Junyi Liu,Jingbin Zeng,Yanli Chen,Weiqing Zhou,Jianguo Liu
标识
DOI:10.1016/j.chroma.2016.02.072
摘要
Dual thermo- and pH-responsive chromatography has been proposed using poly(N-isopropylacrylamide-co-butyl methacrylate-co-N,N-dimethylaminopropyl acrylamide) (P(NIPAM-co-BMA-co-DMAPAAM)) brushes grafted gigaporous polystyrene microspheres (GPM) as matrix. Atom transfer radical polymerization (ATRP) initiator was first coupled onto GPM through Friedel–Crafts acylation with 2-bromoisobutyryl bromide. The dual-responsive polymer brushes were then grafted onto GPM via surface-initiated ATRP. The surface composition, gigaporous structure, protein adsorption and dual-responsive chromatographic properties of the matrix (GPM-P(NIPAM-co-BMA-co-DMAPAAM) were characterized in detail. Results showed that GPM were successfully grafted with thermoresponsive cationic polymer brushes and that the gigaporous structure was well maintained. A column packed with GPM-P(NIPAM-co-BMA-co-DMAPAAM presented low backpressure, good permeability and appreciable thermo-responsibility. By changing pH of the mobile phase and temperature of the column in turn, the column can separate three model proteins at the mobile phase velocity up to 2528 cm h−1. A separation mechanism of this matrix was also proposed. All results indicate that the dual thermo- and pH-responsive chromatography matrix has great potentials in ‘green’ high-speed protein chromatography.
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