生物发光
光蛋白
荧光
激发态
能量转移
发光
生物物理学
绿色荧光蛋白
化学
分子
光化学
生物化学
生物
材料科学
光电子学
物理
化学物理
光学
原子物理学
有机化学
基因
作者
James G. Morin,J. Woodland Hastings
标识
DOI:10.1002/jcp.1040770305
摘要
Abstract Many (but not all) of the bioluminescent systems in coelenter‐ates involve energy transfer from an excited product molecule of the calcium activated photoprotein to a second species, the green fluorescent protein, with emission at 508 nm from its excited state. Although all the luminescent coelen‐terates studied possess photoproteins, not all of them have the green fluorescent protein. This green fluorescent molecule is localized in the luminescent cells; they can thus be easily distinguished by fluorescence microscopy. The active components occur in subcellular particles; these have been isolated in an active form by homogenization in isotonic (to sea water) salt solutions.
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