铁载体
化学
生物合成
皮奥弗丁
肠杆菌素
细菌
生物化学
铁色素
食品科学
作者
Denise M. Burnside,Nicholas P. Cianciotto
出处
期刊:Methods of Molecular Biology
日期:2019-01-01
卷期号:1921: 3-19
标识
DOI:10.1007/978-1-4939-9048-1_1
摘要
This chapter describes the methods for inducing, detecting, and purifying the Legionella pneumophila siderophore. The first protocol details the methods by which L. pneumophila is cultured to facilitate production of the siderophore, rhizoferrin. This chapter then describes how to purify siderophore from culture supernatants through sequential reversed-phase/weak-anion exchange chromatography and high-performance liquid chromatography. The next section describes assays which allow the detection of the iron-binding capability and the biological activity of the purified siderophore. Lastly, this chapter describes the growth of L. pneumophila in chemically defined liquid medium (CDM) containing various iron sources as a method to assess the iron requirements of L. pneumophila.
科研通智能强力驱动
Strongly Powered by AbleSci AI