肠炎沙门氏菌
适体
沙门氏菌
拉曼光谱
表面增强拉曼光谱
化学
色谱法
分析化学(期刊)
细菌
分子生物学
生物
拉曼散射
遗传学
光学
物理
作者
Lei Jin,Shuai Wang,Qing Shao,Yiyu Cheng
标识
DOI:10.1016/j.saa.2021.120625
摘要
Salmonella should be absence in pharmaceutical preparations and foods according to regulations in many countries. Up to now, rapidly detecting Salmonella at 1 CFU·[10 g (mL) ]-1 in pharmaceutical preparation or 1 CFU·[25 g (mL) ]-1 in food samples is still a challenge. Herein, we present an aptamer-based surface-enhanced Raman spectroscopy (SERS) method for rapidly detecting Salmonella Enteritidis by using a handheld Raman instrument. The aptamer could specifically recognize S. Enteritidis, and 4-MBA self-assembled on the surface of Au@Ag NPs was used as a Raman reporter molecule. The method was validated to be high specific with no interference from other five pathogenic bacteria. It could identify S. Enteritidis contaminant at ∼ 1 CFU·(10 g)-1 spiked level in a real sample (Wenxin granule, a botanical drug) after 6 h of enrichment. The detection time was much shorter than that of the methods (more than 54 ∼ 96 h) in the standards of pharmaceutical preparations and foods. In addition, the method could quantitatively determinate S. Enteritidis with satisfactory results. The SERS peak intensities of 4-MBA at 1072 cm-1 showed a good linear correlation (R2 = 0.9873) with the logarithms of S. Enteritidis concentrations ranging from 4.17 × 102 to 1.39 × 107 CFU·mL-1. T-test result (P = 0.425) revealed that there was no significant difference between the determination results obtained by the SERS method and the plate counting method. Therefore, the study indicated that the method was practical and reliable, and it could be a promising alternative for the on-site detection of S. Enteritidis.
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