细胞凋亡
膜联蛋白
癌症研究
生物
免疫印迹
细胞生长
分子生物学
细胞
癌细胞
细胞培养
癌症
程序性细胞死亡
细胞周期
生物化学
遗传学
基因
作者
Tae-Ho Kang,Goo Yoon,In-A Kang,Han-Mo Oh,Jung‐Il Chae,Jung‐Hyun Shim
摘要
Licochalcone B (Lico B), which is normally isolated from the roots of Glycyrrhiza inflata (Chinese Licorice), generally classified into organic compounds including retrochalcones. Potential pharmacological properties of Lico B include anti‐inflammatory, anti‐bacterial, anti‐oxidant, and anti‐cancer activities. However, its biological effects on melanoma and squamous cell carcinoma (SCC) are unknown. Based on these known facts, this study investigated the role of Lico B in apoptosis, through the extrinsic and intrinsic pathways and additional regulation of specificity protein 1 in human skin cancer cell lines. Annexin V/7‐aminoactinomycin D staining, western blot analysis, mitochondrial membrane potential assay, and an anchorage‐independent cell transformation assay demonstrated that Lico B treatment of human melanoma and SCC cells significantly inhibited cell proliferation and induced apoptotic cell death. More specifically, Lico B induced apoptosis through the regulation of specificity protein 1 and apoptosis‐related proteins including CCAAT/enhancer‐binding protein homologous protein, death receptors, and poly (ADP‐ribose) polymerase. These results indicate that Lico B has apoptotic effect on A375 and A431 skin cancer cells, suggesting the potential value of Lico B for the treatment of human melanoma and SCC. Copyright © 2017 John Wiley & Sons, Ltd.
科研通智能强力驱动
Strongly Powered by AbleSci AI