The Gene Control Mechanism of Verenalization in Cyclotella asterocostata and Microcysis aeruginosa

Verenalization in Cyclotella asterocostata and Microcystis aeruginosa was verified in the experiment, and the cause of outbreak is becoming the focus of the study. By analyzing the single cell biological principles of gene manipulation,control of genetic transcription and replication, temperature on the role of the protein and activation process, the gene control mechanism of microalgae vernalization was studied, and the gene controlling model composed of operon, gene regulation and gene activation was established, which is the first time to propose algal vernalization control mechanism of activating gene-operating gene chain model manipulation. The model explains large-scale outbreak mechanism of two kinds of algae in late spring and early summer. In spring, original regulated protein kinase activated by regulatory proteins was produced by regulating protein gene, to combine with genetic manipulation, with starting structure synthesis of DNA synthetase gene transcription and complete DNA replication, algae fission(reproductive) population grew; while in summer, sensitive regulatory protein would activate enzyme denaturation in high temperature, adjust fibrinogen activation of allosteric regulatory protein, with combination of manipulation of genes and activate gene, activate gene cannot activate regulating protein kinase of fibrinogen to produce activated protein, then the growth of Microcystis aeruginosa was inhibited and Cyclotella asterocostata was in the state of high temperature dormancy. In autumn, high temperature thermal denaturation of activating enzyme complex would activate the normal regulatory proteins binding to the operator, with starting structure gene replication of DNA enzymes and suitable temperature and light, Cyclotella asterocostata would not grow, and Microcystis aeruginosa grew but with lower growth rate than that in early summer and later spring. In winter, low temperature heat would denatureactivated enzyme renaturation and activate fibrinogen, a new year's growth was launched in the next year.