合成生物学
代谢工程
生物制品
代谢途径
生化工程
温室气体
原材料
模块化设计
化学
乙醛酸循环
有机化学
酶
生物技术
生物
生物燃料
计算生物学
计算机科学
生态学
工程类
操作系统
作者
Katia Tarasava,Seung Hwan Lee,Jing Chen,Michael Köpke,Michael C. Jewett,Ramón González
出处
期刊:Journal of Industrial Microbiology & Biotechnology
[Oxford University Press]
日期:2022-02-26
卷期号:49 (2)
被引量:15
摘要
Microbial production of fuels, chemicals, and materials has the potential to reduce greenhouse gas emissions and contribute to a sustainable bioeconomy. While synthetic biology allows readjusting of native metabolic pathways for the synthesis of desired products, often these native pathways do not support maximum efficiency and are affected by complex regulatory mechanisms. A synthetic or engineered pathway that allows modular synthesis of versatile bioproducts with minimal enzyme requirement and regulation while achieving high carbon and energy efficiency could be an alternative solution to address these issues. The reverse β-oxidation (rBOX) pathways enable iterative non-decarboxylative elongation of carbon molecules of varying chain lengths and functional groups with only four core enzymes and no ATP requirement. Here, we describe recent developments in rBOX pathway engineering to produce alcohols and carboxylic acids with diverse functional groups, along with other commercially important molecules such as polyketides. We discuss the application of rBOX beyond the pathway itself by its interfacing with various carbon-utilization pathways and deployment in different organisms, which allows feedstock diversification from sugars to glycerol, carbon dioxide, methane, and other substrates.
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