Evaluation of hemolysis, lipemia, and icterus interference with common clinical immunoassays

溶血 胆红素 色谱法 免疫分析 化学 同型半胱氨酸 分析器 甘油三酯 内生 放射免疫分析 血红蛋白 内科学 胆固醇 医学 免疫学 生物化学 抗体
作者
Amir Karin,Victoria Higgins,Jessica Miller,Davor Brinc,Vathany Kulasingam,Rajeevan Selvaratnam
出处
期刊:Clinical Chemistry and Laboratory Medicine [De Gruyter]
卷期号:61 (6): 1035-1045 被引量:14
标识
DOI:10.1515/cclm-2022-0924
摘要

Hemolysis, icterus, and lipemia (HIL) are common sources of endogenous interference in clinical laboratory testing. Defining the threshold of interference for immunoassays enables appropriate reporting of their results when they are affected by HIL.Pools of residual patient serum samples were spiked with a known amount of interferent to create samples with varying concentrations of hemolysate, bilirubin, and Intralipid that mimicked the effects of endogenous HIL. Samples were analysed on the Alinity i analyser (Abbott Diagnostics) for more than 25 immunoassays. The average recovery relative to the non-spiked sample was calculated for each interference level and was compared to a predefined allowable bias.C-peptide, estradiol, serum folate, free T4, homocysteine, insulin, and vitamin B12 were found to be affected by hemolysis, at hemoglobin concentrations between 0.3 to 20 g/L. Immunoassays for BNP, estradiol, free T3, and homocysteine were affected by icterus at conjugated bilirubin concentrations between 50 to 1,044 μmol/L. BNP, serum folate, and homocysteine were affected by Intralipid with measured triglyceride concentrations between 0.8 to 10 mmol/L. Lastly, serological immunoassays for HIV and hepatitis A, B and C were also affected by interferences.Immunoassays are impacted by varying degrees of HIL interference. Some measurands, in the presence of interference, are affected in a manner not previously indicated. The data presented herein provide an independent evaluation of HIL thresholds and will be of aid to resource-limited clinical laboratories that are unable to internally verify endogenous interferences when implementing the Alinity i analyser.
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