Glucose oxidase loaded thermosensitive hydrogel as an antibacterial wound dressing

Skin infection is a worldwide issue that requires joint efforts to develop suitable wound dressing. Hydrogel as the ideal form of wound dressing usually suffered from complicated preparation, low reproducibility and high toxicity/cost. Therefore, the development of cost effective and biocompatible materials to conveniently construct hydrogel based wound dressing in a facile and reproducible way should be one of the future orientation in this field. Here in this study, we proposed a simple thermosensitive hydrogel composed of cost effective and biocompatible Pluronic F-68 and Pluronic F-127, entitled F68/F127 hydrogel. The F68/F127 hydrogel was further loaded with glucose oxidase (GOx@F68/F127 hydrogel) as an antibacterial wound dressing, which showed preferable sol-gel transformation property upon application to the wounds with powerful antibacterial effects. In this way, GOx@F68/F127 hydrogel showed promising inhibition of both Gram positive and negative strains in vitro and in vivo. The antibacterial rate of GOx@F68/F127 reached 100% for Staphylococcus aureus at 0.65 μg/mL and 100% for Escherichia coli at 0.6 μg/mL. And it has high biocompatibility and the ability to promote wound healing. At the end of treatment, mice in the GOx@F68/F127 group had 98% wound healing relative to the control group, which had 30% wound healing. Therefore, GOx@F68/F127 hydrogel can be applied as a promising antimicrobial wound excipient. GOx@F68/F127 hydrogel was prepared for the bacterial infection wound healing.