抗氧化剂
化学
消化(炼金术)
阿布茨
碳酸钙-2
体外
色谱法
特罗洛克
生物化学
吸收(声学)
寡肽
DPPH
肽
物理
声学
作者
Yuan Zhao,Linfan Shi,Zhongyang Ren,Peixiao Wei,Wuyin Weng
摘要
Summary After abalone muscle hot water extract (AHE) was digested in‐vitro , three components (F1, F2, and F3) were separated using the Sephadex G‐15 column, and the antioxidant activity and absorption across Caco‐2 cells were analysed. The result showed that the half‐maximal inhibitory concentrations of F2 for scavenging hydroxyl and ABTS radicals were 0.763 and 0.028 mg mL −1 , respectively, which were lower than those of F1 and F3. Moreover, the Trolox equivalent antioxidant capacity was increased after the F2 was transported through the Caco‐2 cell‐monolayered Transwell chamber. The mass spectrometry analysis revealed that the transported samples contained oligopeptides (Leu‐Tyr, Gly‐Ala‐Ala, and His‐Gly‐Ser‐Ala), and the Leu‐Tyr displayed the highest antioxidant activity and transport rate. Overall, these results suggest that oligopeptides with high antioxidant capacities can be produced from AHE through a combination of in‐vitro digestion and absorption across Caco‐2 cells.
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