纳米技术
生物分子
纳米载体
计算机科学
生命系统
内化
活细胞
人工智能
材料科学
化学
细胞
生物
纳米颗粒
生物系统
生物化学
作者
Jing Zheng,Xiaoxing Fang,Liping Li,Ruiping Zhang,Chao Li
标识
DOI:10.1016/j.trac.2023.117387
摘要
Modern biomedical research has gone deep into the molecular level of cells, trying to reveal the molecular changes of cell function under physiological and pathological conditions. Fluorescent nanoprobes have become a powerful tool for analyzing biomolecules and biological reactions in living cells because of their high stability, enhanced brightness, non-invasive, controllable operation, and easy internalization. In this review, we focus on the design, fabrication and use of fluorescent nanoprobes to detect biomolecules in living cells. We briefly describe the main elements of nanoprobes, such as nanocarriers, recognition elements, and signal transduction strategies. In addition, we also introduce the representative biomolecule-responsive nanoprobes and analyze their advantages and disadvantages in practical applications. It is hoped that this review will stimulate discussion on the construction of next-generation nanoprobes for intelligent and precise detection in living cells, as well as the opportunities and challenges of translating these tools into clinical use.
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