Alternative Structural Lipids Impact mRNA-Lipid Nanoparticle Morphology, Stability, and Activity

activities. Hexagonal and cubic phase structures enhanced mRNA-LNP stability at refrigerated and room temperatures for over six months and three months, respectively. These novel mRNA-LNPs provided promising benefits by reducing immunostimulatory cytokines, warranting further exploration. Our efforts demonstrate the nuances of LNP composition and its effects on structure, stability, and activity.