Nanozyme‐Integrated 3D‐Printed Gradient Scaffold Rescues Redox Homeostasis for Enhanced Osteochondral Repair

脚手架 化学 细胞生物学 组织工程 再生(生物学) 软骨 体内 体外 再生医学 平衡 自愈水凝胶 干细胞 内生 活性氧 关节软骨修复 关节软骨 骨关节炎 生物物理学 生物医学工程 支架蛋白 解剖 骨髓 软骨下骨
作者
Chuan Hu,Dingqi Xie,Jianyi Li,Jin Yang,Jiechao Xia,Gu Jin,Lin Ye,Zhijun Hu
出处
期刊:Small structures [Wiley]
卷期号:7 (1)
标识
DOI:10.1002/sstr.202500413
摘要

Osteochondral defects pose a significant clinical challenge due to their limited capacity for self‐repair. Although arthroplasty is commonly performed, serious complications remain a major concern. In this study, a novel ultrasmall Prussian blue (USPB) nanozyme‐functionalized 3D‐printed gradient antioxidant scaffold was fabricated. Osteogenic and antisenescence hydrogels were incorporated into the subchondral and cartilage regions, respectively. The scaffold's performance, including biocompatibility, effects on cellular senescence, osteogenic differentiation, and underlying mechanisms, was evaluated through a series of in vitro and in vivo assays. The composite scaffold effectively scavenged reactive oxygen species (ROS) and enhanced the endogenous antioxidant defense system in both C28 chondrocytes and rat bone marrow stem cells (rBMSCs). Mechanistically, the cartilage region of the scaffold maintained cellular homeostasis by upregulating GPX4 expression, inhibiting ferroptosis, and bolstering antisenescence activity. In the subchondral region, the scaffold promoted osteogenic differentiation and mineralization of rBMSCs by activating the PI3K‐AKT signaling pathway. This study presents a biomimetic scaffold that demonstrates significant potential for osteochondral repair while systematically elucidating the mechanisms governing cellular homeostasis. These findings provide a foundation for the development of next‐generation multifunctional tissue engineering platforms.
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