微泡
多巴胺能
牙髓干细胞
旁分泌信号
细胞生物学
多巴胺
干细胞
细胞凋亡
神经干细胞
生物
神经发生
化学
神经科学
生物化学
小RNA
基因
受体
作者
Akvilė Jarmalavičiūtė,Virginijus Tunaitis,Ugnė Pivoraitė,Algirdas Venalis,Augustas Pivoriūnas
出处
期刊:Cytotherapy
[Elsevier]
日期:2015-07-01
卷期号:17 (7): 932-939
被引量:187
标识
DOI:10.1016/j.jcyt.2014.07.013
摘要
Stem cells derived from the dental pulp of human exfoliated deciduous teeth (SHEDs) have unique neurogenic properties that could be potentially exploited for therapeutic use. The importance of paracrine SHED signaling for neuro-regeneration has been recognized, but the exact mechanisms behind these effects are presently unknown. In the present study, we investigated the neuro-protective potential of exosomes and micro-vesicles derived from SHEDs on human dopaminergic neurons during oxidative stress-induced by 6-hydroxy-dopamine (6-OHDA).ReNcell VM human neural stem cells were differentiated into dopaminergic neurons and treated with 100 μmol/L of 6-OHDA alone or in combination with exosomes or micro-vesicles purified by ultracentrifugation from SHEDs cultivated in serum-free medium under two conditions: in standard two-dimensional culture flasks or on laminin-coated micro-carriers in a bioreactor. Real-time monitoring of apoptosis was performed with the use of time-lapse confocal microscopy and the CellEvent Caspase-3/7 green detection reagent.Exosomes but not micro-vesicles derived from SHEDs grown on the laminin-coated three-dimensional alginate micro-carriers suppressed 6-OHDA-induced apoptosis in dopaminergic neurons by approximately 80% throughout the culture period. Strikingly, no such effects were observed for the exosomes derived from SHEDs grown under standard culture conditions.Our results suggest that exosomes derived from SHEDs are considered as new potential therapeutic tool in the treatment of Parkinson's disease.
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