诱导多能干细胞
HEK 293细胞
胚胎干细胞
荧光
细胞生理学
多路复用
荧光蛋白
细胞
细胞生物学
生物
绿色荧光蛋白
计算机科学
生物物理学
物理
基因
生物化学
电信
量子力学
作者
Christopher A. Werley,Stefano Boccardo,Alessandra Rigamonti,Emil M. Hansson,Adam E. Cohen
标识
DOI:10.1038/s41467-020-17607-5
摘要
Abstract Cells typically respond to chemical or physical perturbations via complex signaling cascades which can simultaneously affect multiple physiological parameters, such as membrane voltage, calcium, pH, and redox potential. Protein-based fluorescent sensors can report many of these parameters, but spectral overlap prevents more than ~4 modalities from being recorded in parallel. Here we introduce the technique, MOSAIC, Multiplexed Optical Sensors in Arrayed Islands of Cells, where patterning of fluorescent sensor-encoding lentiviral vectors with a microarray printer enables parallel recording of multiple modalities. We demonstrate simultaneous recordings from 20 sensors in parallel in human embryonic kidney (HEK293) cells and in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), and we describe responses to metabolic and pharmacological perturbations. Together, these results show that MOSAIC can provide rich multi-modal data on complex physiological responses in multiple cell types.
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