软骨发生
间充质干细胞
硫氧化物9
干细胞移植修复关节软骨
分子生物学
干细胞
细胞外基质
透明软骨
软骨
骨髓
转染
化学
细胞生物学
移植
生物
II型胶原
细胞分化
免疫学
病理
细胞培养
成体干细胞
解剖
基因表达
医学
内科学
基因
生物化学
遗传学
替代医学
骨关节炎
关节软骨
作者
Hiroki Tsuchiya,Hiroshi Kitoh,Fumiaki Sugiura,Naoki Ishiguro
标识
DOI:10.1016/s0006-291x(02)03026-7
摘要
We investigated chondrogenesis of cell-mediated sox9 gene therapy as a new treatment regimen for cartilage regeneration. pIRES2-EGFP vector containing a full-length mouse sox9 cDNA was transfected into bone marrow-derived mesenchymal stem cells (MSCs) by lipofection and chondrogenic differentiation of these cells was evaluated. In vitro high density micromass culture of these sox9 transfected MSCs demonstrated that a matrix-rich micromass aggregate with EGFP expressing MSCs was positively stained by Alcian blue and type II collagen. Next, sox9 transfected MSCs were loaded into the diffusion chamber and transplanted into athymic mice to analyze in vivo chondrogenesis. A massive tissue formation in about 2mm diameter was visible in the chamber after 4 weeks transplantation. Histological examinations demonstrated that both Alcian blue and type II collagen were positively stained in the extracellular matrix of the mass while type X collagen was not stained. These results indicated that cell-mediated sox9 gene therapy could be a novel strategy for hyaline cartilage damage.
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