结核分枝杆菌
肺结核
生物标志物
蛋白质组学
医学
蛋白质阵列分析
免疫系统
免疫学
折叠变化
生物
病理
下调和上调
生物化学
DNA微阵列
基因表达
基因
作者
Dandan Xu,Danfeng Deng,Xiang Li,Li‐Liang Wei,Yanyuan Li,Xinying Yang,Wei Yu,Chong Wang,Tingting Jiang,Zhongjie Li,Zhongliang Chen,Xing Zhang,Jiyan Liu,Zepeng Ping,Yunqing Qiu,Jicheng Li
出处
期刊:Proteomics
[Wiley]
日期:2014-01-10
卷期号:14 (2-3): 322-331
被引量:67
标识
DOI:10.1002/pmic.201300383
摘要
Pulmonary tuberculosis (TB) caused by Mycobacterium tuberculosis is a chronic disease. Currently, there are no sufficiently validated biomarkers for early diagnosis of TB infection. In this study, a panel of potential serum biomarkers was identified between patients with pulmonary TB and healthy controls by using iTRAQ‐coupled 2D LC‐MS/MS technique. Among 100 differentially expressed proteins screened, 45 proteins were upregulated (>1.25‐fold at p < 0.05) and 55 proteins were downregulated (<0.8‐fold at p < 0.05) in the TB serum. Bioinformatics analysis revealed that the differentially expressed proteins were related to the response to stimulus, the metabolic and immune system processes. The significantly differential expression of apolipoprotein CII (APOCII), CD5 antigen‐like (CD5L), hyaluronan‐binding protein 2 (HABP2), and retinol‐binding protein 4 (RBP4) was further confirmed using immunoblotting and ELISA analysis. By forward stepwise multivariate regression analysis, a panel of serum biomarkers including APOCII, CD5L, and RBP4 was obtained to form the disease diagnostic model. The receiver operation characteristic curve of the diagnostic model was 0.98 (sensitivity = 93.42%, specificity = 92.86%). In conclusion, APOCII, CD5L, HABP2, and RBP4 may be potential protein biomarkers of pulmonary TB. Our research provides useful data for early diagnosis of TB.
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