生物
细胞生物学
免疫学
细胞
电池类型
受体
遗传学
作者
Michal Cohen,Amir Giladi,Anna-Dorothea Gorki,Dikla Gelbard Solodkin,Mor Zada,Anastasiya Hladik,András G. Miklósi,Tomer-Meir Salame,Keren Bahar Halpern,Eyal David,Shalev Itzkovitz,Tibor Harkany,Sylvia Knapp,Ido Amit
出处
期刊:Cell
[Elsevier]
日期:2018-11-01
卷期号:175 (4): 1031-1044.e18
被引量:322
标识
DOI:10.1016/j.cell.2018.09.009
摘要
Lung development and function arises from the interactions between diverse cell types and lineages. Using single-cell RNA sequencing (RNA-seq), we characterize the cellular composition of the lung during development and identify vast dynamics in cell composition and their molecular characteristics. Analyzing 818 ligand-receptor interaction pairs within and between cell lineages, we identify broadly interacting cells, including AT2, innate lymphocytes (ILCs), and basophils. Using interleukin (IL)-33 receptor knockout mice and in vitro experiments, we show that basophils establish a lung-specific function imprinted by IL-33 and granulocyte-macrophage colony-stimulating factor (GM-CSF), characterized by unique signaling of cytokines and growth factors important for stromal, epithelial, and myeloid cell fates. Antibody-depletion strategies, diphtheria toxin-mediated selective depletion of basophils, and co-culture studies show that lung resident basophils are important regulators of alveolar macrophage development and function. Together, our study demonstrates how whole-tissue signaling interaction map on the single-cell level can broaden our understanding of cellular networks in health and disease.
科研通智能强力驱动
Strongly Powered by AbleSci AI