Comparison of Microbiological Results of Two Bone Sampling Methods in Diabetic Foot Osteomyelitis—Debridement vs. Operation

The purpose of this study is to compare the microbiological results of debridement bone samples with those of corresponding results of operation bone samples for patients with diabetic foot osteomyelitis, using 16s rRNA high-throughput sequencing approach. We prospectively recruited 24 diabetic foot patients with osteomyelitis, and obtained 9 bone samples during surgery(Ds-Group) and 16 bone samples from debridement(Dd-Group). We compared the differences between debridement and operation procedures based on microbiological study of bone samples via 16s rRNA sequencing, using Ion PGM™ System Sequencer. It was obvious that microbes in Ds-Group, with narrower distribution, were more stable in alpha and beta diversity analysis. As for genera, totally 14 dominant genera were separated, and the average of Ds-Group and Dd-Group were respectively 10.44 and 9.44, with no statistically significant difference(P>0.05). Halomonas spp. and Prevotella spp. were the predominant genera of Ds-Group and Dd-Group respectively. Ds-Group had significant more Anaerococcus spp., Dialister spp. and Pseudomonas spp. than Dd-Group(P<0.05), but less Halomonas spp. and Nesterenkonia spp. than Dd-Group(P<0.05). There were no difference in other dominant genera, such as Bacteroides spp., Finegoldia spp., Fusobacterium spp., Parvimonas spp., Prevotella spp., Proteus spp., Staphylococcus spp., Streptococcus spp. and Veillonella spp.(P>0.05). We might draw a conclusion that though surgical operation was a little bit more accurate than debridement, microbiological study of bone samples from debridement could be capable enough for the diagnosis and treatment guidance of diabetic foot osteomyelitis. Disclosure M. Zou: None. P. Hu: None. Y. Pan: None. X. Luo: None. Y. Jiang: None. Y. Xue: None. Y. Cao: None. F. Gao: None.