生物
病毒复制
核糖核酸
甲型流感病毒
抄写(语言学)
长非编码RNA
RNA干扰
反义RNA
病毒学
干扰素
转录因子
细胞生物学
基因表达
病毒
基因
分子生物学
遗传学
哲学
语言学
作者
Qi Wang,Daining Zhang,Wenjing Feng,Yidi Guo,Xiaoning Sun,Maolin Zhang,Zhenhong Guan,Ming Duan
摘要
Abstract Although the expression of thousands of host long noncoding RNAs (lncRNAs) can be regulated by viral infection, the number of lncRNAs with experimentally verified function is limited. In this study, the expression of host lncRNA TSPOAP1‐AS1 was significantly induced by influenza A virus (IAV) infection in a dose‐ and time‐dependent manner. Polyinosine‐polycytidylic acid (poly (I:C)), a synthetic analog of double‐stranded RNA, also increased TSPOAP1‐AS1 expression. RNA fractionation revealed that TSPOAP1‐AS1 was a nucleocytoplasmic lncRNA, and an increased nuclear/cytoplasmic ratio was detected after IAV infection. The nuclear factor‐κB signaling acting as a critical factor in the transcription of TSPOAP1‐AS1 was determined through the use of pharmacological and genetic approaches. Functionally, overexpression of TSPOAP1‐AS1 resulted in a significant increase in IAV replication. In contrast, the abolition of TSPOAP1‐AS1 by RNA interference restricted viral replication. Furthermore, we demonstrated that TSPOAP1‐AS1 negatively modulated the IAV‐induced Ifnb1 transcription, interferon‐sensitive response element (ISRE) activation, and downstream interferon‐stimulated genes expression. Collectively, our data provides evidence for the host lncRNA utilized by viruses to support its replication.
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