玉米赤霉烯酮
黄曲霉毒素
化学
色谱法
真菌毒素
检出限
分子生物学
生物
食品科学
作者
Xianfeng Ren,Qi Zhang,Wenhua Wu,Tingting Yan,Xiaoqian Tang,Wen Zhang,Li Yu,Peiwu Li
出处
期刊:Food Chemistry
[Elsevier]
日期:2019-11-01
卷期号:297: 124912-124912
被引量:34
标识
DOI:10.1016/j.foodchem.2019.05.186
摘要
An anti-idiotypic nanobody-phage display-mediated immuno-polymerase chain reaction (PD-IPCR) method was developed for simultaneous quantitative detection of total aflatoxins and zearalenone in cereals. Two phages, displaying the variable domain of the heavy chain anti-idiotypic nanobody that binds aflatoxin- or zearalenone-specific monoclonal antibody (1C11 or 2D3), were used as competitors for corresponding analytes. Specific DNA sequences encoding anti-idiotypic nanobodies were used to design the primers for PCR amplification. The results indicated that detection limits for total aflatoxins and zearalenone in a sample were 0.03 and 0.09 ng mL-1, respectively. Recoveries of spiked aflatoxins and zearalenone were 80-118% and 76.7-111%, respectively. Validation results were in good agreement with the gold-standard high-performance liquid chromatography method. This report is the first to describe PD-IPCR for simultaneous quantitative detection of total aflatoxins and zearalenone in cereals.
科研通智能强力驱动
Strongly Powered by AbleSci AI