Culture of Dorsal Root Ganglion Neurons in Embryonic Rat

Objective To establish a culture system of dorsal root ganglion neurons derived from embryonic rat.Method Dorsal root ganglion neurons harvested from E16 rats were produced into single cell suspension,then cultured dorsal root ganglion neurons.The purificational rates were evaluated according to neuronal specific enolase(NSE) immunocytochemistry stain.Result Cultured dorsal root ganglion neurons could survive for about 1 month and extend the process which formed dense net work.The neuron purity was high.The purification rate of neurons was about 90%.Conclusion Our experiment has established an easy,practical,economic method for the culture system of dorsal root ganglion neurons.It is a useful model for further studies.