大肠杆菌
聚合酶
RNA聚合酶
琼脂糖
特异性因子
DNA
化学
酶
分子生物学
核糖核酸
色谱法
RNA聚合酶Ⅱ
RNA依赖性RNA聚合酶
生物化学
生物
基因
基因表达
发起人
作者
Majid Mehrpouyan,W. Scott Champney
出处
期刊:Journal of Biochemical and Biophysical Methods
[Elsevier]
日期:1990-11-01
卷期号:21 (4): 289-297
被引量:1
标识
DOI:10.1016/0165-022x(90)90004-v
摘要
A method is described for the rapid purification of RNA polymerase holoenzyme from small amounts of Escherichia coli cells. Chromatography of a crude extract on a single-stranded DNA agarose column followed by gell filtration chromatography gave 95% pure holoenzyme. The enzyme kinetic characteristics on T7 DNA identical to those of RNA polymerase purified by other more laborious procedures.
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