外消旋化
化学
二肽
半抗原
水解
催化作用
色氨酸
酰胺
苯丙氨酸
氨基酸
肽
缬氨酸
肽合成
膦酸盐
亮氨酸
立体化学
单克隆抗体
抗体
有机化学
生物化学
生物
免疫学
作者
Ralph Hirschmann,Amos B. Smith,Carol M. Taylor,Patricia A. Benkovic,Scott D. Taylor,Kraig M. Yager,Paul A. Sprengeler,Stephen J. Benkovic
出处
期刊:Science
[American Association for the Advancement of Science]
日期:1994-07-08
卷期号:265 (5169): 234-237
被引量:350
标识
DOI:10.1126/science.8023141
摘要
Monoclonal antibodies, induced with a phosphonate diester hapten, catalyzed the coupling of p -nitrophenyl esters of N -acetyl valine, leucine, and phenylalanine with tryptophan amide to form the corresponding dipeptides. All possible stereoisomeric combinations of the ester and amide substrates were coupled at comparable rates. The antibodies did not catalyze the hydrolysis of the dipeptide product nor hydrolysis or racemization of the activated esters. The yields of the dipeptides ranged from 44 to 94 percent. The antibodies were capable of multiple turnovers at rates that exceeded the rate of spontaneous ester hydrolysis. This achievement suggests routes toward creating a small number of antibody catalysts for polypeptide syntheses.
科研通智能强力驱动
Strongly Powered by AbleSci AI