紫杉醇
透明质酸
细胞毒性
CD44细胞
体内
药理学
化学
体外
MTT法
药代动力学
脂质体
癌细胞
阳离子聚合
癌症
生物化学
医学
生物
有机化学
生物技术
内科学
解剖
作者
Xiaoyan Yang,Yunxia Li,Min Li,Li Zhang,Lixia Feng,Na Zhang
出处
期刊:Cancer Letters
[Elsevier]
日期:2013-07-01
卷期号:334 (2): 338-345
被引量:207
标识
DOI:10.1016/j.canlet.2012.07.002
摘要
The aim of our study was to develop hyaluronic acid-coated, paclitaxel-loaded, nanostructured lipid carriers (HA-NLCs) prepared via electrostatic attraction for delivering paclitaxel (PTX) to tumors overexpressing CD44. First, cationic PTX-NLC was prepared by melt emulsion technology. Then, PTX-NLC were coated with hyaluronic acid (HA). The in vitro release of PTX was evaluated by the dialysis method. This analysis showed that PTX was released more slowly from HA-NLC than from Taxol®. The in vitro cytotoxicity of HA-NLC was investigated using the MTT method in B16, CT26 and HCT116 cell lines. The results showed that the cytotoxicity of HA-NLC against these three cancer cell lines was superior to that of Taxol®. The in vivo antitumor effect, the pharmacokinetics and the tissue distribution of HA-NLC were all evaluated in B16-bearing Kunming mice. The results showed that HA-NLC was better tolerated and had increased antitumor activity in B16-bearing Kunming mice compared with Taxol®. Furthermore, HA-NLC could prolong the circulation time of PTX in blood and increase the accumulation of PTX in the tumor. Therefore, HA-NLC prepared via electrostatic attraction was an effective carrier for delivering PTX to tumors overexpressing CD44.
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