等离子体子
逆转录酶
注意事项
聚合酶链反应
2019年冠状病毒病(COVID-19)
多路复用
纳米颗粒
病毒学
纳米技术
材料科学
逆转录聚合酶链式反应
严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)
2019-20冠状病毒爆发
化学
光电子学
计算机科学
医学
信使核糖核酸
生物化学
爆发
病理
基因
护理部
电信
传染病(医学专业)
疾病
作者
Nicole R. Blumenfeld,Michael Anne E. Bolene,Martin A. Jaspan,Abigail G. Ayers,Sabin Zarrandikoetxea,Juliet Freudman,Nikhil Shah,Angela M. Tolwani,Yuhang Hu,Terry L. Chern,James Rogot,Vira Behnam,Aditya Sekhar,Xinyi Liu,Bulent Onalir,Robert Kasumi,Abdoulaye Sanogo,Kelia Human,Kasey Murakami,Goutham S. Totapally,Mark J. Fasciano,Samuel K. Sia
标识
DOI:10.1038/s41565-022-01175-4
摘要
Quantitative polymerase chain reaction (qPCR) offers the capabilities of real-time monitoring of amplified products, fast detection, and quantitation of infectious units, but poses technical hurdles for point-of-care miniaturization compared with end-point polymerase chain reaction. Here we demonstrate plasmonic thermocycling, in which rapid heating of the solution is achieved via infrared excitation of nanoparticles, successfully performing reverse-transcriptase qPCR (RT-qPCR) in a reaction vessel containing polymerase chain reaction chemistry, fluorescent probes and plasmonic nanoparticles. The method could rapidly detect SARS-CoV-2 RNA from human saliva and nasal specimens with 100% sensitivity and 100% specificity, as well as two distinct SARS-CoV-2 variants. The use of small optical components for both thermocycling and multiplexed fluorescence monitoring renders the instrument amenable to point-of-care use. Overall, this study demonstrates that plasmonic nanoparticles with compact optics can be used to achieve real-time and multiplexed RT-qPCR on clinical specimens, towards the goal of rapid and accurate molecular clinical diagnostics in decentralized settings. Quantitative polymerase chain reaction allows the real-time detection of nucleic acids in human samples, representing a gold standard for infection detection, but it cannot be easily converted into a point-of-care approach. Here a strategy is proposed to leverage plasmonic polymerase chain reaction to achieve multiplexed, fluorescence detection of SARS-CoV-2 RNA from human saliva and nasal specimen, showing promise as a point-of-care approach.
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