生物
外胚层
胚胎干细胞
胚胎发生
胚泡
EZH2型
卵母细胞
表观遗传学
母子转换
胚胎
细胞生物学
遗传学
男科
作者
Yinan Zhao,Dandan Bai,You Wu,Dan Zhang,Mengying Liu,Yingpu Tian,Jinhua Lu,Haibin Wang,Shaorong Gao,Zhongxian Lu
出处
期刊:Development
[The Company of Biologists]
日期:2022-07-07
卷期号:149 (15)
摘要
ABSTRACT How maternal Ezh1 and Ezh2 function in H3K27 methylation in vivo in pre-implantation embryos and during embryonic development is not clear. Here, we have deleted Ezh1 and Ezh2 alone or simultaneously from mouse oocytes. H3K27me3 was absent in oocytes without Ezh2 alone, while both H3K27me2 and H3K27me3 were absent in Ezh1/Ezh2 (Ezh1/2) double knockout (KO) oocytes. The effects of Ezh1/2 maternal KO were inherited in zygotes and early embryos, in which restoration of H3K27me3 and H3K27me2 was delayed by the loss of Ezh2 alone or of both Ezh1 and Ezh2. However, the ablation of both Ezh1 and Ezh2, but not Ezh1 or Ezh2 alone, led to significantly decreased litter size due to growth retardation post-implantation. Maternal Ezh1/2 deficiency caused compromised H3K27me3 and pluripotent epiblast cells in late blastocysts, followed by defective embryonic development. By using RNA-seq, we examined crucial developmental genes in maternal Ezh1/2 KO embryos and identified 80 putatively imprinted genes. Maternal Ezh1/2-H3K27 methylation is inherited in offspring embryos and has a critical effect on fetal and placental development. Thus, this work sheds light on maternal epigenetic modifications during embryonic development.
科研通智能强力驱动
Strongly Powered by AbleSci AI