顺铂
姜黄素
周细胞
封堵器
氧化应激
药理学
紧密连接
周围神经病变
细胞毒性
病理
医学
癌症研究
化学
内分泌学
内皮干细胞
内科学
生物化学
化疗
体外
糖尿病
作者
Phetnarin Kobutree,Amornrat Tothonglor,Atitaya Roumwong,Depicha Jindatip,Sithiporn Agthong
出处
期刊:Folia Morphologica
[VM Media Sp zo.o. - VMGroup SK]
日期:2023-08-29
卷期号:82 (3): 533-542
被引量:4
标识
DOI:10.5603/fm.a2022.0065
摘要
Cisplatin is a platinum-based antineoplastic agent used to treat cancers of solid organs. Neuropathy is one of its major side effects, necessitating dose reduction or cessation. Previous studies suggested that cisplatin causes microvascular toxicity, including pericyte detachment. This study aimed to clarify whether these alterations occurred in the blood-nerve barrier (BNB) of capillaries after cisplatin treatment.Electron microscopic analysis of rat sciatic nerves with cisplatin neuropathy showed increased frequency and severity of pericyte detachment. Moreover, the vascular basement membrane did not tightly encircle around the endothelial cells and pericytes. Cultured human umbilical vein endothelial cells and human brain vascular pericytes showed reduced viability, increased caspase-3 activity and enhanced oxidative stress following cisplatin treatment. In addition, cisplatin decreased transendothelial electrical resistance (TEER) and the expression of the tight junction proteins occludin and zonula occludens-1. Curcumin, a polyphenol found in the root of Curcuma longa, had favourable effects on cisplatin neuropathy in previous work. Therefore, curcumin was tested to determine whether it had any effect on these abnormalities. Curcumin alleviated pericyte detachment, cytotoxicity, oxidative stress, TEER reduction and tight junction protein expression.These data indicate that cisplatin causes BNB disruption in the nerves and might result in neuropathy. Curcumin might improve neuropathy via the restoration of BNB. Whether alterations in the BNB occur and curcumin is effective in patients with cisplatin neuropathy remain to be investigated.
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